Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia
Loading...
Date
2024-10-14
Authors
Jimenez, Diego Javier
Chaparro, Dayanne
Sierra, Felipe
Custer, Gordon F.
Feuerriegel, Golo
Chuvochina, Maria
Diaz-García, Laura
Mendes, Lucas William
Ortega Santiago, Yina Paola
Rubiano-Labrador, Carolina
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Mangroves are impacted by multiple environmental stressors, including sea level
rise, erosion, and plastic pollution. Thus, mangrove soil may be an excellent source
of as yet unknown plastic-transforming microorganisms. Here, we assess the
impact of polyethylene terephthalate (PET) particles and seawater intrusion on the
mangrove soil microbiome and report an enrichment culture experiment to artifi-
cially select PET-transforming microbial consortia. The analysis of metagenomeassembled genomes of two bacterial consortia revealed that PET catabolism can
be performed by multiple taxa, of which particular species harbored putative
novel PET-active hydrolases. A key member of these consortia (Mangrovimarina
plasticivorans gen. nov., sp. nov.) was found to contain two genes encoding
monohydroxyethyl terephthalate hydrolases. This study provides insights into the
development of strategies for harnessing soil microbiomes, thereby advancing
our understanding of the ecology and enzymology involved in microbial-mediated
PET transformations in marine-associated systems.
Description
Keywords
Citation
Jiménez, D. J., Chaparro, D., Sierra, F., Custer, G. F., Feuerriegel, G., Chuvochina, M., ... & Rosado, A. S. (2024). Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia. Trends in Biotechnology.