Chavarro Mesa, EdissonHerrera-Blanco, Néstor AndrésBeltrán-Acosta, Camilo RubénCotes-Prado, Alba MarinaÁngel-Díaz, Jorge Evelio2022-03-182022-03-182021-10-182022-03-18Chavarro-Mesa, E., Herrera-Blanco, N. E., Beltrán-Acosta, C. R., Cotes-Prado, A. M., & Ángel-Díaz, J. E. (2021). Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes. Ciencia y TecnologíaAgropecuaria, 22(3), e1888. https://doi.org/10.21930/rcta.vol22_num3_art:1888https://hdl.handle.net/20.500.12585/10632Stem canker and black scurf are potato diseases caused by the fungus Rhizoctonia solani, anastomosisgroup 3 (AG-3PT). This fungus affects potato roots, stems, and tubers, reducing crop yields. This study aimsto determine the genetic diversity of R. solaniAG-3PT obtained from the Colombian departments of Antioquia, Boyacá, and Cundinamarca. Restrictionfragment lengthpolymorphism(RFLP) analysis of the ITS-5.8S ribosomal DNA region allowed the differentiation and specific identification of AG-3PT and AG2-1 anastomosis groups. These results confirm that AG-3PT is the primary causative agent and etiologicalorigin of stem canker and black scurf in Colombia. Two distinct subgroups within R. solaniAG-3PT were identified using random amplified microsatellite markers (RAMs); AG-3PT (A) shares a similarity index of 78%, and AG-3PT (B) shares a similarity of 79% among its isolates. These subgroups were not linked to a geographical origin but to the AG group to which they belong. Nei’s genetic diversity [D] of 0.25 confirmed the high genetic diversity for AG-3PT through RAMsanalysis, related to a high evolutive potential within the AG-3PT group in Colombia. Finally, the R. solaniAG-3PT fungus obtained from Cundinamarca had the adaptative potential to emerge as a pathogen of Solanum phurejain Colombia, possibly due tothe similarity between pathosystems.24 Páginasapplication/pdfenghttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/articlehttps://doi.org/10.21930/rcta.vol22_num3_art:1888Random amplified microsatellites (RAMs)Ribosomal DNASolanum phurejaSolanum tuberosumThanatephorus cucumerisinfo:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 InternacionalUniversidad Tecnológica de BolívarRepositorio Universidad Tecnológica de Bolívar